4CNM
Crystal structure of human 5T4 (Wnt-activated inhibitory factor 1, Trophoblast glycoprotein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.310, 67.310, 96.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.940 - 1.750 |
| R-factor | 0.18365 |
| Rwork | 0.182 |
| R-free | 0.22153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.833 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.810 |
| Number of reflections | 33062 | |
| <I/σ(I)> | 17.9 | 3.1 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 9.6 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | THE CRYSTALLIZATION DROP CONTAINED 100 NL OF CONCENTRATED 5T4 (5.5 MG/ML), 100 NL OF 25% W/V POLYETHYLENE GLYCOL (PEG) 3350, 0.1 M CITRATE PH 3.5, AND 0.5 NL OF 0.1 M NAOH |
