4CNC

Crystal structure of human 5T4 (Wnt-activated inhibitory factor 1, Trophoblast glycoprotein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-26
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	49.600, 95.820, 65.970
Unit cell angles	90.00, 91.14, 90.00

Refinement procedure

Resolution	54.330 - 1.770
R-factor	0.17929
Rwork	0.177
R-free	0.21727
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5T4 CRYSTAL FORM WITH 1 MOLECULE IN THE ASYMMETRIC UNIT
RMSD bond length	0.016
RMSD bond angle	1.905
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.330	1.820
High resolution limit [Å]	1.770	1.770
Rmerge	0.060	0.760
Number of reflections	59830
<I/σ(I)>	13.8	2.5
Completeness [%]	99.7	99.5
Redundancy	5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			THE CRYSTALLIZATION SITTING DROP CONTAINED 100 NL OF CONCENTRATED 5T4/WAIF1 (5 MG/ML IN 20 MM TRIS-HCL, PH 7.5, 150 MM NACL) PLUS 100 NL OF 0.2 M (NH4)2SO4, 30 % W/V PEG 4000.