4CL7
Crystal structure of VEGFR-1 domain 2 in presence of Cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.746, 41.711, 94.694 |
| Unit cell angles | 87.05, 82.25, 73.03 |
Refinement procedure
| Resolution | 19.469 - 2.000 |
| R-factor | 0.1707 |
| Rwork | 0.168 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.983 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.470 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.330 |
| Number of reflections | 26362 | |
| <I/σ(I)> | 10.74 | 3.01 |
| Completeness [%] | 97.1 | 96.6 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | CRYSTAL WERE OBTAINED BY HANGING DROP METHOD FROM 1MM VEGFR1-D2, 5MM HEPES/NAOH PH7.0, 10MM COCL2, 100 MM BIS-TRIS/HCL PH6.5 15%(W/V) PEG3350. |
