4CKK
Apo structure of 55 kDa N-terminal domain of E. coli DNA gyrase A subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.570, 95.530, 95.890 |
| Unit cell angles | 105.16, 118.81, 103.42 |
Refinement procedure
| Resolution | 81.510 - 1.900 |
| R-factor | 0.17031 |
| Rwork | 0.169 |
| R-free | 0.18859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.510 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 194509 | |
| <I/σ(I)> | 11 | 2.7 |
| Completeness [%] | 97.0 | 94.7 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
