4CKK
Apo structure of 55 kDa N-terminal domain of E. coli DNA gyrase A subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-14 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 93.570, 95.530, 95.890 |
Unit cell angles | 105.16, 118.81, 103.42 |
Refinement procedure
Resolution | 81.510 - 1.900 |
R-factor | 0.17031 |
Rwork | 0.169 |
R-free | 0.18859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y3p |
RMSD bond length | 0.013 |
RMSD bond angle | 1.502 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.510 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.490 |
Number of reflections | 194509 | |
<I/σ(I)> | 11 | 2.7 |
Completeness [%] | 97.0 | 94.7 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |