4CKC
Vaccinia virus capping enzyme complexed with SAH (monoclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.710, 200.260, 128.100 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 125.000 - 2.900 |
| R-factor | 0.25697 |
| Rwork | 0.255 |
| R-free | 0.29706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.400 |
| Number of reflections | 62825 | |
| <I/σ(I)> | 5.05 | 2.13 |
| Completeness [%] | 92.0 | 81.1 |
| Redundancy | 1.88 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN SOLUTION AT 4 MG/ML IN 40 MM TRIS-HCL, PH 8.0, 200 MM NACL, 5 MM DTT WITH 5 MM ADOMET. RESERVOIR SOLUTION: 0.1 M TRIS-HCL, PH 7.5, 0.2 M LICL, 0.2M (NH4)2SO4, 15% PEG4000 |
