4CID
Structural insights into the N-terminus of the EHD2 ATPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-24 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.851, 134.653, 56.068 |
Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
Resolution | 78.150 - 3.000 |
R-factor | 0.23255 |
Rwork | 0.230 |
R-free | 0.27917 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qpt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.027 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.050 | 0.340 |
Number of reflections | 26426 | |
<I/σ(I)> | 10.5 | 1.8 |
Completeness [%] | 95.9 | 98 |
Redundancy | 1.63 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100 MM MES PH 6.5, 20 MM MGCL2, 4% PEG3350, 5% MPD |