4CFE
Structure of full length human AMPK in complex with a small molecule activator, a benzimidazole derivative (991)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PIXEL |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.030, 134.141, 140.558 |
Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
Resolution | 19.914 - 3.023 |
R-factor | 0.2194 |
Rwork | 0.218 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2Y94 2f15 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.653 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.210 |
High resolution limit [Å] | 3.020 | 3.020 |
Rmerge | 0.040 | 0.440 |
Number of reflections | 50545 | |
<I/σ(I)> | 21 | 2.2 |
Completeness [%] | 93.4 | 95 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | CRYSTALS WERE GROWN BY THE HANGING DROP METHOD WITH RESERVOIR SOLUTION CONTAINING 13% PEG3350, 0.1M MGCL2, 1% GLUCOSE, 0.15% CAPB IN 0.1M IMIDAZOLE AT PH 6.2. |