4CEI
Crystal structure of ADPNP-bound AddAB with a forked DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.944, 151.335, 124.560 |
Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
Resolution | 29.949 - 2.800 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u44 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.749 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (PHASER-MR) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.930 | 2.860 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 68664 | |
<I/σ(I)> | 15.9 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 0.1M HEPES PH 7.5, 17.5 % PEG 1500, 0.25M NACL, 0.1M SODIUM FORMATE, 0.1M MGCL2, VAPOR DIFFUSION, HANGING DROP, 285K |