4CE3
Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.180, 74.180, 110.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.450 - 2.310 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ah6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.450 | 2.370 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 13834 | |
| <I/σ(I)> | 17.5 | 2.5 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 5.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
