4CCK
60S ribosomal protein L8 histidine hydroxylase (NO66) in complex with Mn(II) and N-oxalylglycine (NOG)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-07 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 101.410, 80.710, 151.540 |
| 格子定数 [度] | 90.00, 94.54, 90.00 |
精密化法
| 残基 | 81.100 - 2.150 |
| R因子 | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4diq |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 81.100 | 2.210 |
| 分解能 [Å] (高) | 2.150 | 2.150 |
| Rmerge_l_obs | 0.080 | 0.660 |
| 独立反射数 | 131084 | |
| <I/σ(I)> | 10.2 | 2.2 |
| 完全性 [%] | 98.9 | 97.9 |
| 冗長性 | 4.5 | 4.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | VAPOR DIFFUSION, SITTING DROP 0.1M BIS-TRIS PROPANE PH 5.6-6.5, 0.5-0.7M MAGNESIUM FORMATE, 0.002M MNCL2, TEMPERATURE 293K |
