4CC4
Complex of InlC of Listeria monocytogenes and human Tuba C-terminal SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-03 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.702, 101.750, 126.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.600 |
| R-factor | 0.19914 |
| Rwork | 0.196 |
| R-free | 0.26187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1XEU 1zuu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.748 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.660 |
| Number of reflections | 36275 | |
| <I/σ(I)> | 21.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.8 M NA2HPO4, 0.8 M K2HPO4, 0.1 M HEPES PH 7.5 |
