4CC0

Notch ligand, Jagged-1, contains an N-terminal C2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-24
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	55.980, 60.250, 62.650
Unit cell angles	92.59, 104.44, 106.67

Refinement procedure

Resolution	30.190 - 2.320
R-factor	0.219
Rwork	0.218
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.190
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.190
High resolution limit [Å]	2.320
Rmerge	0.140
Number of reflections	31717
<I/σ(I)>	4.3
Completeness [%]	97.3
Redundancy	1.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1