4CBW
Crystal structure of Plasmodium berghei actin I with D-loop from muscle actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.240, 69.530, 178.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.581 - 2.501 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.160 | 1.500 |
| Number of reflections | 24163 | |
| <I/σ(I)> | 9.2 | 0.9 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 6.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS-HCL (PH 8.0), 8% (W/V), PEG 20000, 2% (V/V) DIOXANE |
