4CBS
X-ray structure of quintuple mutant of human alanine glyoxylate aminotransferase, AGXT_RHEAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.610, 89.610, 142.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.741 - 2.300 |
R-factor | 0.2226 |
Rwork | 0.221 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yob |
RMSD bond length | 0.008 |
RMSD bond angle | 1.208 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.740 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.350 |
Number of reflections | 26518 | |
<I/σ(I)> | 23.1 | 8.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.5 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 291 | 15% PEG 10K; 0.1 M HEPES PH 7.5, 2.5% V/V ISOPROPANOL AND 5% GLYCEROL AS ADDITIVE. |