4CBS

X-ray structure of quintuple mutant of human alanine glyoxylate aminotransferase, AGXT_RHEAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 21 2
Unit cell lengths	89.610, 89.610, 142.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.741 - 2.300
R-factor	0.2226
Rwork	0.221
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yob
RMSD bond length	0.008
RMSD bond angle	1.208
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.740	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.350
Number of reflections	26518
<I/σ(I)>	23.1	8.2
Completeness [%]	100.0	100
Redundancy	14.5	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	15% PEG 10K; 0.1 M HEPES PH 7.5, 2.5% V/V ISOPROPANOL AND 5% GLYCEROL AS ADDITIVE.