4CBM
Pestivirus NS3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.100, 159.585, 99.227 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.270 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.980 | 3.450 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rmerge | 0.130 | 0.550 |
| Number of reflections | 31533 | |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 94.4 | 91.7 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M NAAC, 0.025 M CALCIUM ACETATE, 9 % PEG 4000, pH 4.6 |
