4CBJ
The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus OF4 is adapted to alkaliphilic cell physiology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-27 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.940, 98.330, 121.930 |
| Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
| Resolution | 48.332 - 2.800 |
| R-factor | 0.2376 |
| Rwork | 0.235 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2v |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.180 | 1.050 |
| Number of reflections | 41447 | |
| <I/σ(I)> | 10.13 | 1.8 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 9.2 | 8.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.4 | pH 4.4 |
