4CBJ
The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus OF4 is adapted to alkaliphilic cell physiology
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-05-27 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.940, 98.330, 121.930 |
Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
Resolution | 48.332 - 2.800 |
R-factor | 0.2376 |
Rwork | 0.235 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x2v |
RMSD bond length | 0.002 |
RMSD bond angle | 0.573 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.180 | 1.050 |
Number of reflections | 41447 | |
<I/σ(I)> | 10.13 | 1.8 |
Completeness [%] | 99.0 | 98.7 |
Redundancy | 9.2 | 8.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.4 | pH 4.4 |