4CBC
Open-form NavMS Sodium Channel Pore (with C-terminal Domain) after thallium soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.901, 331.704, 79.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.469 - 2.664 |
| R-factor | 0.2772 |
| Rwork | 0.276 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BHA |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.933 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.470 | 2.790 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.300 | 0.800 |
| Number of reflections | 30860 | |
| <I/σ(I)> | 11.7 | 2.7 |
| Completeness [%] | 99.5 | 96.2 |
| Redundancy | 19.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M NA3CITRATE, 0.1 M TRIS, PH 8.0, 34% PEG400 |
