4C9H
Structure of yeast mitochondrial ADP/ATP carrier isoform 2 inhibited by carboxyatractyloside (P212121 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.354, 98.203, 105.061 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.839 - 3.200 |
| R-factor | 0.2506 |
| Rwork | 0.249 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9g |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 3.460 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.190 | 0.610 |
| Number of reflections | 11939 | |
| <I/σ(I)> | 6.9 | 2.4 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 10MM SODIUM CITRATE, 50MM AMMONIUM SULFATE, 8% T-BUTANOL, 23.5% PEG600. |
