4C9G
Structure of yeast mitochondrial ADP/ATP carrier isoform 2 inhibited by carboxyatractyloside (C2221 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.900, 105.670, 98.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.047 - 2.490 |
| R-factor | 0.2608 |
| Rwork | 0.259 |
| R-free | 0.29730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1okc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.907 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.050 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.090 | 0.900 |
| Number of reflections | 12639 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 10MM SODIUM CITRATE, 8% T-BUTANOL, 28% PEG600, 0.2% (W/V) NONYL GLUCOSIDE |
