4C93
Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Pol alpha.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 88.977, 99.998, 219.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.205 - 2.694 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.20990 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 2.770 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.160 | 1.470 |
| Number of reflections | 54802 | |
| <I/σ(I)> | 10.2 | 1.4 |
| Completeness [%] | 99.6 | 95 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M TRI-SODIUM CITRATE PH 6.2, 7-9% PEG 8000 |
