4C8Q
Crystal structure of the yeast Lsm1-7-Pat1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 258.687, 258.687, 47.103 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.420 - 3.698 |
| R-factor | 0.2513 |
| Rwork | 0.249 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c92 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.533 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.680 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.070 | 0.690 |
| Number of reflections | 36441 | |
| <I/σ(I)> | 11.3 | 1.4 |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE |
