4C7R
Inward facing conformation of the trimeric betaine transporter BetP in complex with lipids
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | MARRESEARCH 165 MM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.560, 129.500, 167.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.421 - 2.700 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wit |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.725 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.200 | |
| Number of reflections | 73562 | |
| <I/σ(I)> | 12.9 | 1.39 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 6.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 291 | 100MM 3NA-CITRATE, 300MM NACL, 20% PEG 400, PH 5.5, 18 DEG CELSIUS |
