4C7O
The structural basis of FtsY recruitment and GTPase activation by SRP RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2011-11-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.720, 166.330, 154.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.222 - 2.600 |
R-factor | 0.1657 |
Rwork | 0.163 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cnw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.220 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.050 | 0.770 |
Number of reflections | 45637 | |
<I/σ(I)> | 13.38 | 2.77 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 4.28 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |