4C7G
Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 120 |
Collection date | 2012-05-19 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.170, 64.450, 143.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.830 - 1.800 |
R-factor | 0.16926 |
Rwork | 0.167 |
R-free | 0.21006 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c7d |
RMSD bond length | 0.019 |
RMSD bond angle | 1.814 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.350 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.510 |
Number of reflections | 50954 | |
<I/σ(I)> | 12.2 | 3.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES PH 7.0, 4 % PEG 6000 |