4C7F
Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 120 |
| Collection date | 2011-07-23 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.790, 129.240, 149.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.890 - 2.000 |
| R-factor | 0.15848 |
| Rwork | 0.156 |
| R-free | 0.19712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c7d |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.180 | 0.530 |
| Number of reflections | 95616 | |
| <I/σ(I)> | 8.8 | 3.2 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 6.5, 4 % (W/V) PEG 6000, 5 % DMSO |
