4C7D
Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.660, 128.900, 150.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.850 - 1.850 |
| R-factor | 0.16465 |
| Rwork | 0.163 |
| R-free | 0.20231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hp4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.888 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.240 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 120786 | |
| <I/σ(I)> | 16.8 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.3, 8 % (W/V) PEG 6000 |
