4C6T

Crystal structure of the RPS4 and RRS1 TIR domain heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-29
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61 2 2
Unit cell lengths	93.359, 93.359, 416.648
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.794 - 2.650
R-factor	0.185
Rwork	0.182
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RPS4 TIR AND RRS1 TIR
RMSD bond length	0.009
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.840	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.100	1.500
Number of reflections	32619
<I/σ(I)>	27.5	2
Completeness [%]	99.9	100
Redundancy	23	24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		1.8 M SODIUM MALONATE PH 6.0