4C5Q
measles virus phosphoprotein tetramerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PIXEL PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.343, 34.392, 139.693 |
| Unit cell angles | 97.04, 96.90, 90.12 |
Refinement procedure
| Resolution | 34.125 - 2.200 |
| R-factor | 0.2522 |
| Rwork | 0.250 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ezj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | PHENIX |
| Phasing software | PHENIX (PHASER) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.870 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 32947 | |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 96.3 | 88.7 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 10% PEG 8000, 0.1 M NACL, 0.1 M CHES, PH 9.5 |
