4C59
Structure of GAK kinase in complex with nanobody (NbGAK_4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.268, 37.753, 75.546 |
| Unit cell angles | 90.00, 111.46, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.800 |
| R-factor | 0.20516 |
| Rwork | 0.202 |
| R-free | 0.26163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3LL6 AND 1OP9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.690 |
| Number of reflections | 11580 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 9% PEG 3350, 0.06 M MAGNESIUM FORMATE, pH 7.5 |
