4C4K
Crystal structure of the titin M10-Obscurin Ig domain 1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.940, 66.880, 72.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.480 - 1.950 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wp3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 15005 | |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 0.2 M SODIUM ACETATE, 30% PEG4000 |
