4C4A
Crystal structure of mouse protein arginine methyltransferase 7 in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.322, 97.322, 168.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.353 - 1.700 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAME STRUCTURE SOLVED BY SAD USING A CRYSTAL OF THE NATIVE PROTEIN SOAKED WITH THIMEROSAL AND PHASED WITH ANOMALOUS SIGNAL FROM MERCURY |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | |
| Number of reflections | 107019 | |
| <I/σ(I)> | 13.9 | 0.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.7 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PEG 20,000 3.2%, PEG 8,000 12.6%, BTP PH 7.0 100 MM, SAH 1 MM, NDSB256 100 MM |
