4C48
Crystal structure of AcrB-AcrZ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 145.010, 145.010, 538.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.670 - 3.300 |
| R-factor | 0.28343 |
| Rwork | 0.281 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dx5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.389 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.460 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | 0.990 |
| Number of reflections | 33271 | |
| <I/σ(I)> | 11 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | CRYSTALLISATION SOLUTION 100 MM HEPES, PH 7.5, 10 MM MGCL2 AND 12% (W/V) PEG 3350. SAMPLE BUFFER 10 MM HEPES PH: 7.5, 50 MM SODIUM CHLORIDE, 0.03% DDM N-DODECYL BETA-D-MALTOPYRANOSIDE |
