4C3T
The Carbonic anhydrase from Thermovibrio ammonificans reveals an interesting intermolecular disulfide contributing to increasing thermal stability of this enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.930, 80.930, 154.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.230 - 1.690 |
R-factor | 0.22284 |
Rwork | 0.221 |
R-free | 0.25175 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kop |
RMSD bond length | 0.007 |
RMSD bond angle | 1.241 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.930 | 1.740 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.070 | 1.230 |
Number of reflections | 734424 | |
<I/σ(I)> | 18.6 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.7 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.5 M LICL, 50 MM SODIUM CITRATE, 10% PEG6000, PH 4.0 |