4C3J
Structure of 14-subunit RNA polymerase I at 3.35 A resolution, crystal form C2-90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 401.020, 139.840, 140.940 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 49.050 - 3.350 |
| R-factor | 0.2134 |
| Rwork | 0.213 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.440 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.190 | 1.500 |
| Number of reflections | 112047 | |
| <I/σ(I)> | 8.48 | 0.87 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% MPD, 0.1M TRIS HCL, PH 7.5 |
