4C3G
cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | DIRECT ELECTRON DE-12 (4k x 3k) |
| Number of Images | 656 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.6 |
| Software | DireX,FREALIGN,Xmipp |
