4C3E
HRSV M2-1 mutant S58D S61D, P21 crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.680, 141.600, 142.160 |
| Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
| Resolution | 78.240 - 2.400 |
| R-factor | 0.20515 |
| Rwork | 0.203 |
| R-free | 0.23769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.120 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 138151 | |
| <I/σ(I)> | 11.1 | 2.4 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 6.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20 % (V/V) PEG MME 2000, 0.2 M TRIMETHYLAMINE N-OXIDE, 0.1 M TRIS PH 8.5 |
