4C3E
HRSV M2-1 mutant S58D S61D, P21 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-10 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.680, 141.600, 142.160 |
Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
Resolution | 78.240 - 2.400 |
R-factor | 0.20515 |
Rwork | 0.203 |
R-free | 0.23769 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.584 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.120 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 138151 | |
<I/σ(I)> | 11.1 | 2.4 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 6.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 20 % (V/V) PEG MME 2000, 0.2 M TRIMETHYLAMINE N-OXIDE, 0.1 M TRIS PH 8.5 |