4C2Y
Human N-myristoyltransferase (NMT1) with Myristoyl-CoA co-factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.740, 178.480, 58.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.240 - 1.640 |
| R-factor | 0.1726 |
| Rwork | 0.170 |
| R-free | 0.22157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iu1 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.260 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.070 | 0.710 |
| Number of reflections | 98990 | |
| <I/σ(I)> | 17.7 | 2.4 |
| Completeness [%] | 98.2 | 85 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 22.5% PEG 4000, 5 MM NICL2, 100 MM NA CITRATE PH 4.5, 2.5% GLYCEROL |
