4C20

L-Fucose Isomerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-13
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	151.931, 151.931, 408.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.070 - 2.410
R-factor	0.15596
Rwork	0.154
R-free	0.19045
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fui
RMSD bond length	0.010
RMSD bond angle	1.397
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.410	2.410
Rmerge	0.080	0.380
Number of reflections	70320
<I/σ(I)>	24.2	6.3
Completeness [%]	99.9	99.9
Redundancy	10.7	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		SPFCSI AT A CONCENTRATION OF 12 MG ML-1, BUFFERED IN 20 MM TRIS-HCL (PH 7.0), 150 MM NACL, AND 2 MM DTT WAS CRYSTALLIZED BY MIXING WITH EQUAL VOLUMES OF 100 MM TRIS-HCL (PH 8.0), 27% (W PER V) POLYETHYLENE GLYCOL 4,000, 50 MM NACL, AND 200 MM MAGNESIUM SULPHATE