4C0R
Molecular and structural basis of glutathione import in Gram-positive bacteria via GshT and the cystine ABC importer TcyBC of Streptococcus mutans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00, 1.55 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.040, 103.120, 64.090 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 37.010 - 1.547 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.18310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q2c |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.692 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 69164 | |
| <I/σ(I)> | 16.21 | 2.89 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.7 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M CADMIUM CHLORIDE HYDRATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH 4.6, 30% V/V POLYETHYLENE GLYCOL 400 |
