4C0Q
Transportin 3 in complex with Ran(Q69L)GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.950, 93.514, 104.713 |
| Unit cell angles | 78.43, 68.29, 68.28 |
Refinement procedure
| Resolution | 39.946 - 3.420 |
| R-factor | 0.2689 |
| Rwork | 0.268 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c0p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.749 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.600 |
| High resolution limit [Å] | 3.420 | 3.420 |
| Rmerge | 0.120 | 1.500 |
| Number of reflections | 34998 | |
| <I/σ(I)> | 8.6 | 1.5 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 13.5% (W/V) PEG3350, 8.5% (W/V) BENZAMIDINE HYDROCHLORIDE, 0.2M SODIUM ACETATE, PH6.5 |
