4C0D

Structure of the NOT module of the human CCR4-NOT complex (CNOT1-CNOT2-CNOT3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-20
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	91.570, 165.920, 78.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.480 - 3.200
R-factor	0.2262
Rwork	0.224
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4C0E 4c0f 4c0g
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	3.280
High resolution limit [Å]	3.200	3.200
Number of reflections	20282
<I/σ(I)>	10.1	2.2
Completeness [%]	98.9	98.9
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M MES PH=6.5, 12% PEG20000