4C0B

Structure of wild-type Clp1p-Pcf11p (454 -563) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	110
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	89.352, 95.410, 182.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.550 - 2.770
R-factor	0.1805
Rwork	0.179
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2npi
RMSD bond length	0.010
RMSD bond angle	1.310
Data reduction software	XDS
Data scaling software	SCALEPACK
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.920
High resolution limit [Å]	2.770	2.770
Rmerge	0.010	0.390
Number of reflections	40274
<I/σ(I)>	13.9	1.9
Completeness [%]	99.7	99.77
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.5