4C0A
Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 90.980, 65.780, 196.860 |
| Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
| Resolution | 42.840 - 3.300 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1R8S AND 3QWM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.030 | 0.090 |
| Number of reflections | 67437 | |
| <I/σ(I)> | 6.8 | 1.94 |
| Completeness [%] | 99.0 | 95.6 |
| Redundancy | 3.24 | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.15 M AMMONIUM SULFATE, 0.1 M MES PH 6 AND 16% PEG 4000 |
