4BZY
Crystal structure of human glycogen branching enzyme (GBE1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.278, 164.538, 311.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.303 - 2.750 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3amk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.759 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.300 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.170 | 0.870 |
| Number of reflections | 78285 | |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% PEG 3350, 0.05M SODIUM SUCCINATE |
