4BZP
Structure of the Mycobacterium tuberculosis APS kinase CysC in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Collection date | 2010-06-19 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.630, 66.330, 61.760 |
Unit cell angles | 90.00, 103.59, 90.00 |
Refinement procedure
Resolution | 22.260 - 1.470 |
R-factor | 0.15954 |
Rwork | 0.158 |
R-free | 0.18187 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pey |
RMSD bond length | 0.016 |
RMSD bond angle | 1.911 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.120 | 1.550 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.070 | 0.070 |
Number of reflections | 55519 | |
<I/σ(I)> | 9.1 | 9.1 |
Completeness [%] | 96.0 | 96 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M NH4NO3 24 % PEG 3,350 25 % ETHYLENE GLYCOL |