4BZ1
Structure of dengue virus EDIII in complex with Fab 3e31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.802, 107.009, 134.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.504 - 2.150 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r29 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.739 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.060 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | |
| Number of reflections | 32253 | |
| <I/σ(I)> | 19.54 | 2.1 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 14 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.01 M ZINC CHLORIDE, 0.1 M SODIUM ACETATE PH 5.0, 20% (W/V) PEG 6000 |
