4BYH
Crystal structure of sialylated IgG Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 153.000, 153.000, 111.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.340 - 2.300 |
| R-factor | 0.22426 |
| Rwork | 0.222 |
| R-free | 0.26165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.501 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.770 |
| Number of reflections | 34456 | |
| <I/σ(I)> | 17.7 | 2.8 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 8.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 4M NACL, 0.1 M CITRATE PH 4.0 |
