4BXO
Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 125.100, 125.100, 74.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.430 - 2.150 |
| R-factor | 0.20615 |
| Rwork | 0.204 |
| R-free | 0.25523 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.983 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BNP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.340 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 32846 | |
| <I/σ(I)> | 11.4 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 18%W/V PEG 3350, 0.2M CALCIUM ACETATE, 0.01M BARIUM CHLORIDE., pH 8.0 |
