4BXN
Eg5(WT) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2011-04-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 81.426, 81.426, 115.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.935 - 2.793 |
| R-factor | 0.2333 |
| Rwork | 0.230 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4as7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4 (SUITE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.800 | 3.000 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 9633 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.02 M CACL2, 0.02 M CADMIUM CHLORIDE, 0.02 M COBALT(II) CHLORIDE, 20% W/V PEG-3350, pH 6.8 |
