4BWQ

Crystal structure of U5-15kD in a complex with PQBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-07
Detector	MARRSEARCH
Spacegroup name	P 1 2 1
Unit cell lengths	77.220, 40.040, 118.770
Unit cell angles	90.00, 93.45, 90.00

Refinement procedure

Resolution	38.540 - 2.100
R-factor	0.1893
Rwork	0.186
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgv
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.520	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.450
Number of reflections	42475
<I/σ(I)>	8.5	2.6
Completeness [%]	98.7	97.3
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1